Development of Blood Flow and Oxygen Monitoring System for Mice Based on Laser Speckle and Spectrum / 中国医疗器械杂志

simultaneous monitoring of blood flow and changes of concentration of oxyhemoglobin (ΔHbO) in brain is a key important method for the research of cerebrovascular disease. In this study, a new monitoring system, combining laser speckle contrast imaging method and spectral analysis method, was proposed, which could be utilized to measure the cerebral blood flow and ΔHbO on mice during traumatic brain injury. The principle of the present system was studied and the hardware platform of the detection system was built. Then, user interface software and algorithms were implemented based on Labview and Matlab software. Finally, the performance of the present system was verified by the experiments.

Development of Diffuse Reflectance Spectroscopy Detection and Analysis System for Cervical Cancer / 中国医疗器械杂志

Cervical cancer is a common female malignant tumor. It has been increasing and rejuvenating in recent years. Early screening of cervical cancer is an effective control method to block cancer. In this study, a diffuse reflectance spectrum detection and analysis system based on LabWindows development software and MariaDB database was developed, which can acquire and save the spectral data to the database. The method of a neural network model based on spectral database was built to distinguish the cervical tissue and the normal tissue. The nude mouse tumor model test and human volunteer test were performed respectively, which verified that the system can distinguish between normal tissue and tumor tissue, and can be applied to the screening of cervical precancerous lesions.

Exploration of teaching approach of medical instrument development course with national defense features / 中华医学教育探索杂志

The aim of the development course in university is to expand the multidisciplinary knowledge.The students,who choose development course,are coming from many subjects.Therefore,the design and teaching methods of this course should have their own characteristics.This study introduces the exploration of medical instrument course with defense features and talent training mode.The core ideas of curriculum reform include the following.The course contents should be professionally subdivided,so that all the students from different majors can obtain the knowledge.Teaching methods should be guided by the national defense requirements using lectures and discussions.Multiply assessment modes,such as speech,discussion,paper poster,and creative exhibition,can be used to evaluate the students.The present reforms have been implemented in the reality of course teaching practice.The reform contents in this paper have a great significance to the creation of development courses based on the integration of preponderant disciplines in the university.

Research on Optical Parameter along Puncture Path in Spinal Surgery Navigation Based on Near Infrared Spectroscopy / 生物医学工程学杂志

Accurate placement of pedicle screws is a key factor of spinal surgery. Investigation of a new real-time intra-operative monitoring method is an important area of clinical application research which makes a contribution to planting pedicle screw accurately. Porcine spines were chosen as experimental objects. The changes of reduced scattering coefficient (μ'(s)) along normal puncture path, medial perforation path and lateral perforation path were measured and studied. A conclusion is drawn that there are two distinct peaks throughout the puncture process, appearing at the junction of cancellous bone and cortical bone, at the beginning and at the end, respectively. The reduced scattering coefficient is proved to be a good monitoring factor which can identify whether the screw is about to reach the critical position of the spine puncture. Moreover, the variation provides an important reference for spinal surgical navigation process.

Study on real-time monitoring and assessment of near-infrared in the dehydration treatment of traumatic brain injury / 生物医学工程学杂志

We used near-infrared spectroscopy technology to monitor and assess the treatment effect of dehydrating agent in injured rat brain in real time style. We employed the brain edema model in rats resulting from Feeney' s freefall damage, then treated with different doses of mannitol, and collected reduced scattering coefficient (p',) and intracranial pressure (ICP) values after the injury and during the treatment. The results showed that brain edema happened 1 h after the injury in rats' brain tissue, peaked around 72 h after injury, and then began to decrease gradually. The reduced scattering coefficient and ICP values of the treatment group injected with mannitol all decreased after administration. Compared with the effect of low-dose mannitol treatment, that of high-dose mannitol treatment was much better. The duration of the plateau was longer and most experiments results declined significantly. From this we conclude that the reduced scattering coefficient and ICP are consistent with the trend changes, and the reduced scattering coefficient could be used as an indicator for monitoring cerebral edema.

Research on an in vivo near-infrared real-time monitoring system and its application in pharmacokinetics / 生物医学工程学杂志

The real-time in vivo measurement method has been urgently needed in the research of pharmacokinetics. In the present paper a new in vivo detection method based on fluorescence spectroscopy has been proposed and the monitoring system has been built which is used for pharmacokinetics studies in rats. The relationship between fluorescence intensity and concentration was obtained. By detecting the fluorescent dye Cypate in real-time in rats, the properties of the system have been validated by comparing with the fluorescence imaging system in vitro. The results showed that the system could be feasible for: (1) The linear regression equation of Cypate concentration in the range of 0.098-25 microg/ml is y = 73.249x + 130.97 (R2 = 0.9991 and P < 0.001). RSD of high, medium and low concentration is 1.23%, 6.29% and 13.48%, respectively, and the detecting sensitivity is 0.0981 g/ml; (2) The fluorescent dye concentration from the system is consistent (r = 0.9925) with the fluorescence imaging system in vitro. The fluorescent dye metabolism in rats can be well detected. It can be concluded that a new real-time in vivo detecting method in the paper can be used in pharmacokinetics research.

The theory and research of near-infrared optical tomography / 生物医学工程学杂志

The fundamental theory and new research of near-infrared optical tomography (NIR OT) were introduced. NIR OT can offer the functional as well as structural information of tissue, so its applications have emerged in areas such as functional imaging of the brain, breast cancer detection, continue monitoring of the brain in babies and any other medical applications. The paper broadly divides the types of NIR OT instruments into three groups: continuous intensity, intensity-modulated and time-resolved instruments. The diffuse function of light propagation through tissue was given, and emphasized on the two distinct solutions to image reconstruction: linear methods based on inverse scattering theory and non-linear methods based on model fitting. Give some advanced work we can do in the future, concurrent NIR OT and other conventional anatomical imaging techniques, such as MRI, CT, research on the presence of anisotropies, contrast agent in NIR OT, three-dimensional image reconstruction. At last enumerate some of the fundamental difficulties involved in NIR OT, such as the intensity matching problem between simulated and experiment data, the boundary effect problem. Although these are often acknowledged, they remain largely unexplored.

Optimization approach to inverse problems in near-infrared optical tomography / 生物医学工程学杂志

In this paper, we introduce an optimization approach to the inverse model of near-infrared optical tomography (NIR OT), which can reconstruct the optical properties, namely the absorption and scattering coefficients of thick tissue such as brain and breast tissues. A modeling and simulation tool, named Femlab and based on finite element methods, has been tested wherein the forward models are based on the diffusion equation. Then the inverse model is soved; this is regarded as an optimization approach, including the tests on difference between the measured data and the predicted data, and the optimization methods of optical properties. The algorithms used for optimization are multi-species Genetic Algorithms based on multi-encoding. At last, the whole strategy for the Femlab and optimization approach is given. The strategy is proved to be sufficient by the simulation results.

Data processing of functional magnetic resonance of brain based on statistical parametric mapping / 生物医学工程学杂志

This paper introduces the data processing of functional magnetic resonance imaging (fMRI) of brain based on statistical parametric mapping (SPM). Because of its excellent spatial resolution and temporal resolution as well as its noninvasive approach with no involvement in the injection of radioactive materials, the fMRI of brain has been widely used by brain researchers in the world. There are four steps in the processing, i. e. reading the raw data, pre-processing, statistical analysis of the data and model estimation. The pre-processing includes motion correction, normalization, spatial smoothing and temporal smoothing. The statistical analysis of the data includes building the general linear model. Model estimation includes serial T-test and inspecting the activation region. In this paper, emphases are laid on the new methods in every processing.